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IMPDH2 antibody

Anti-IMPDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101778
Anti-IMPDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101778
Anti-IMPDH2 antibody used in Western Blot (WB). GTX101778
Anti-IMPDH2 antibody used in Western Blot (WB). GTX101778

Cat. No. GTX101778

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IMPDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IMPDH2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240979

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inosine monophosphate dehydrogenase 2 , IMPD2 , IMPDH-II

Background

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IMPDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101778

GTX101778 IHC-P Image

IMPDH2 antibody detects IMPDH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
IMPDH2 antibody (GTX101778) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IMPDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101778

GTX101778 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using IMPDH2(GTX101778) antibody at 1:500 dilution.

Anti-IMPDH2 antibody used in Western Blot (WB). GTX101778

GTX101778 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101778 diluted at 1:1000

Anti-IMPDH2 antibody used in Western Blot (WB). GTX101778

GTX101778 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX101778 diluted at 1:1000

REFERENCE

Application Reference

Kim SH et al. Breast Cancer Res Treat 2016; 157 (1) : 41-54 Withaferin A inhibits in vivo growth of breast cancer cells accelerated by Notch2 knockdown.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169