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Iba1 antibody

Orthogonal
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042
Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Flow cytometry (FACS). GTX100042

Cat No. GTX100042

Reference 24
2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all Iba1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , mouse brain , mouse liver , rat liver , *Mouse brain

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Allograft Inflammatory Factor 1,Aif-1,Iba1,Irt-1,Irt1,Aif1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Iba1 antibody detects Iba1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
15 % SDS-PAGE
Iba1 antibody (GTX100042) dilution: 1:1000
Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.
Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042

GTX100042 IHC-Fr Image

Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100042

GTX100042 ICC/IF Image

Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:400.
Blue: Hoechst 33342 staining.
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1(GTX100042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Iba1 antibody detects Iba1 in the cytosol of Kupffer cell in human hepatoma by immunohistochemical analysis.

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma stroma.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain tissue.
Iba1 (GTX100042) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
The anti-Iba1 (GTX100042) specifically recognizes Iba1, a microglia marker, in murine brain tissues.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Orthogonal

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-Iba1 antibody used in Flow cytometry (FACS). GTX100042

GTX100042 FACS Image

Iba1 antibody (GTX100042) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX100042) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
REFERENCE

Application Reference

G?tzl JK et al. EMBO Mol Med 2019; 11 (6) Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism.
Application : WB
Reactivity : Mouse
Kamarehei M et al. Brain Res Bull 2019; 147 : 174-182 Increased expression of endoplasmic reticulum stress-related caspase-12 and CHOP in the hippocampus of EAE mice.
Application : IHC-P
Reactivity : Mouse
Wahl D et al. Cell Rep 2018; 25 (8) : 2234-2243.e6 Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice.
Application : IHC-Fr
Reactivity : Mouse
Huang YR et al. Neurobiol Dis 2018; 124 : 202-217 Naturally occurring autoantibodies against α-synuclein rescues memory and motor deficits and attenuates α-synuclein pathology in mouse model of Parkinson's disease.
Application : IHC-P
Reactivity : Mouse
Josephine Juettner et al. bioRxiv 2018; (Epub) Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates, and humans.
Application : IHC-Fr
Reactivity : Human
Show More +
Submit a Reference
REVIEW

Iba1 antibody Cat No. GTX100042

Rating
( Average 4.5 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 4 based on 1 users reviews)
Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042
Date : Anonymous submitted on 05-Jun-2018
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : Brain
Fixation : 4% PFA, 4°C, 48Hr
Primary Antibody : 1:100, 4°C, overnightHr
Anti-Iba1 antibody used in Immunohistochemistry (IHC). GTX100042
Date : Anonymous submitted on 18-Mar-2013
Score :
Application Tested : IHC
Sample Species : Ms
Sample : Mouse brain
Blocking : NGS,BSA,Trition X-100, 25°C, 2Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.1M citrate buffer(pH6.0), 95°C, 10minHr
Primary Antibody : 1:1000, 25°C, 18Hr
Submit a Review
Application Reference
G?tzl JK et al. EMBO Mol Med 2019; 11 (6) Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism.
Application : WB
Reactivity : Mouse
Kamarehei M et al. Brain Res Bull 2019; 147:174-182 Increased expression of endoplasmic reticulum stress-related caspase-12 and CHOP in the hippocampus of EAE mice.
Application : IHC-P
Reactivity : Mouse
Wahl D et al. Cell Rep 2018; 25 (8):2234-2243.e6 Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice.
Application : IHC-Fr
Reactivity : Mouse
Huang YR et al. Neurobiol Dis 2018; 124:202-217 Naturally occurring autoantibodies against α-synuclein rescues memory and motor deficits and attenuates α-synuclein pathology in mouse model of Parkinson's disease.
Application : IHC-P
Reactivity : Mouse
Josephine Juettner et al. bioRxiv 2018; (Epub) Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates, and humans.
Application : IHC-Fr
Reactivity : Human
Jhan MK et al. J Leukoc Biol 2018; (Epub) Anti-TNF-α restricts dengue virus-induced neuropathy.
Application : IHC-Fr
Reactivity : Mouse
Ji M et al. Alzheimers Res Ther 2018; 10 (1):55 Hepatitis B core VLP-based mis-disordered tau vaccine elicits strong immune response and alleviates cognitive deficits and neuropathology progression in Tau.P301S mouse model of Alzheimer's disease and frontotemporal dementia.
Application : IHC-P
Reactivity : Mouse
Liu SY et al. Brain Res 2018; (Epub) Intravenous immunoglobulin ameliorates motor and cognitive deficits and neuropathology in R6/2 mouse model of Huntington's disease by decreasing mutant huntingtin protein level and normalizing NF-κB signaling pathway.
Huang WY et al. J Neuroinflammation 2018; 15 (1):140 NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation.
Application : WB
Reactivity : Mouse
Hashiguchi T et al. Drug Metab Dispos 2018; (Epub) Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.
Application : ICC/IF
Reactivity : Mouse
Huang YN et al. Sci Rep 2018; 8 (1):2368 Neuroprotective effects of pifithrin-α against traumatic brain injury in the striatum through suppression of neuroinflammation, oxidative stress, autophagy, and apoptosis.
Application : IHC
Reactivity : Rat
Souza LC et al. Mol Cell Neurosci 2018; 88:93-106 Aging exacerbates cognitive and anxiety alterations induced by an intracerebroventricular injection of amyloid-β1-42 peptide in mice.
Application : IHC-P
Reactivity : Mouse
Lin W et al. Cell Transplant 2017; 26 (11):1798-1810 Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages.
Application : IHC-P
Reactivity : Rat
Ingold I et al. Cell 2017; (Epub) Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis.
Application : IHC-Fr
Reactivity : Mouse
Lin HJ et al. Cell Physiol Biochem 2017; 44 (5):1726-1740 Gamma-Secretase Inhibitors Attenuate Neurotrauma and Neurogenic Acute Lung Injury in Rats by Rescuing the Accumulation of Hypertrophic Microglia.
Application : IHC-P
Reactivity : Rat
Huang N et al. Oncotarget 2017; 8 (46):81001-81013 PLGA nanoparticles modified with a BBB-penetrating peptide co-delivering Aβ generation inhibitor and curcumin attenuate memory deficits and neuropathology in Alzheimer's disease mice.
Application : WB, IHC-P
Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10):e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Stroobants S et al. Neurobiol Dis 2017; Long-term enzyme replacement therapy improves neurocognitive functioning and hippocampal synaptic plasticity in immune-tolerant alpha-mannosidosis mice.
Application : IHC-Fr
Reactivity : Mouse
Wang SW et al. Alzheimers Res Ther 2017; 9 (1):41 A vaccine with Aβ oligomer-specific mimotope attenuates cognitive deficits and brain pathologies in transgenic mice with Alzheimer's disease.
Application : IHC-Fr
Reactivity : Mouse
Wolf H et al. Dis Model Mech 2016; 9 (9):1015-28 A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease.
Application : ICC/IF
Reactivity : Mouse
Zha J et al. Sci Rep 2016; 6:36631 A scFv antibody targeting common oligomeric epitope has potential for treating several amyloidoses.
Application : IHC-P
Reactivity : Mouse
Liu HS et al. Sci Rep 2016; 6:19137 Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway.
Kowalewski B et al. Hum Mol Genet 2015; 24 (7):1856-68 Ataxia is the major neuropathological finding in arylsulfatase G-deficient mice: similarities and dissimilarities to Sanfilippo disease (mucopolysaccharidosis type III).
Application : IHC
Reactivity : Mouse
Kowalewski B et al. Hum. Mol. Genet. 2014; 24 (7):1856-68 Ataxia is the major neuropathological finding in Arylsulfatase G deficient mice: Similarities and dissimilarities to Sanfilippo disease (Mucopolysaccharidosis type III).
Application : IHC-Fr
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169