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Iba1 antibody

Comparison Orthogonal
Anti-Iba1 antibody used in Western Blot (WB). GTX101495
Anti-Iba1 antibody used in Western Blot (WB). GTX101495
Anti-Iba1 antibody used in Western Blot (WB). GTX101495
Anti-Iba1 antibody used in Flow cytometry (FACS). GTX101495
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Anti-Iba1 antibody used in Immunoprecipitation (IP). GTX101495
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Anti-Iba1 antibody used in Flow cytometry (FACS). GTX101495
Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101495
Anti-Iba1 antibody used in Western Blot (WB). GTX101495
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Anti-Iba1 antibody used in Western Blot (WB). GTX101495

Cat. No. GTX101495

Reference 19
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240433

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody used in Western Blot (WB). GTX101495

GTX101495 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody (GTX101495) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Iba1 antibody used in Western Blot (WB). GTX101495

GTX101495 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody (GTX101495) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Iba1 antibody used in Western Blot (WB). GTX101495

GTX101495 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Iba1 antibody used in Flow cytometry (FACS). GTX101495

GTX101495 FACS Image

Iba1 antibody (GTX101495) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX101495) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in Immunoprecipitation (IP). GTX101495

GTX101495 IP Image

Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5 μg of Iba1 antibody (GTX101495).
Western blot analysis was performed using Iba1 antibody (GTX101495).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in Flow cytometry (FACS). GTX101495

GTX101495 FACS Image

Flow cytometry on primary murine microglia cells, staining with Iba1 (GTX101495) antibody using 1.0 μg per 4×105 cells. GTX101495 (blue), Rabbit IgG (green) ,Unstained (red).

Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101495

GTX101495 ICC/IF Image

Iba1 antibody detects Iba1 protein at cell periphery by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody used in Western Blot (WB). GTX101495

GTX101495 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in Western Blot (WB). GTX101495

GTX101495 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

E Partiot et al. EMBO Mol Med 2024; Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies
Sun XY et al. Signal Transduct Target Ther 2023; 8 (1) : 30 Fc effector of anti-Aβ antibody induces synapse loss and cognitive deficits in Alzheimer's disease-like mouse model.
Li J et al. Front Pharmacol 2022; 13 : 940999 Therapeutic effects of total saikosaponins from Radix bupleuri against Alzheimer's disease.
Chung-Min Tsai et al. Neurophotonics 2022; 9 (1) : 015006 Transcranial photobiomodulation (808?nm) attenuates pentylenetetrazole-induced seizures by suppressing hippocampal neuroinflammation, astrogliosis, and microgliosis in peripubertal rats.
Xiao-Ying Sun et al. J Neuroinflammation 2021; 18 (1) : 131 Rutin prevents tau pathology and neuroinflammation in a mouse model of Alzheimer's disease.
Application : WB, ICC/IF, IHC-P
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Iba1 antibody Cat. No. GTX101495

Rating
( Average 4.5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101495
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Brain tissue
Fixation : 4% PFA, 4°C, 48Hr
Primary Antibody : 1:100, 4°C, 16Hr
Anti-Iba1 antibody used in Flow cytometry (FACS). GTX101495
Date : Anonymous submitted on 20-Jan-2013
Score :
Application Tested : FACS
Sample Species : Ms
Sample : Primary murine microglia
Blocking : 2.5% horse serum in 1X PBS, 4°C, 1Hr
Fixation : 4% paraformaldehyde + 0.2% triton X-100
Primary Antibody : 1: 1.0 μg in 4×10*5 cells, 4°C, 1Hr
Figure Note : GTX101495 (blue), Rabbit IgG (green) ,Unstained (red)
Submit a Review
Application Reference
E Partiot et al. EMBO Mol Med 2024; Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies
Sun XY et al. Signal Transduct Target Ther 2023; 8 (1):30 Fc effector of anti-Aβ antibody induces synapse loss and cognitive deficits in Alzheimer's disease-like mouse model.
Li J et al. Front Pharmacol 2022; 13:940999 Therapeutic effects of total saikosaponins from Radix bupleuri against Alzheimer's disease.
Chung-Min Tsai et al. Neurophotonics 2022; 9 (1):015006 Transcranial photobiomodulation (808?nm) attenuates pentylenetetrazole-induced seizures by suppressing hippocampal neuroinflammation, astrogliosis, and microgliosis in peripubertal rats.
Xiao-Ying Sun et al. J Neuroinflammation 2021; 18 (1):131 Rutin prevents tau pathology and neuroinflammation in a mouse model of Alzheimer's disease.
Application : WB, ICC/IF, IHC-P
Reactivity : Mouse
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
Application : IHC-P
Navarro-Sempere A et al. Mediators Inflamm 2021; 2021:5891095 The Health Hazards of Volcanoes: First Evidence of Neuroinflammation in the Hippocampus of Mice Exposed to Active Volcanic Surroundings.
Application : IHC-P
Ho MH et al. Redox Biol 2021; 46:102067 CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury.
Application : IHC-Fr
Reactivity : Mouse
Lhussiez V et al. Invest Ophthalmol Vis Sci 2020; 61 (11):18 Cohen Syndrome-Associated Cataract Is Explained by VPS13B Functions in Lens Homeostasis and Is Modified by Additional Genetic Factors.
Application : WB
Reactivity : Mouse
Baskaran R et al. J Comp Neurol 2019; (Epub) Characterization of striatal phenotypes in heterozygous Disc1 mutant mice, a model of haploinsufficiency.
Application : IHC
Reactivity : Mouse
xu miao et al. Molecular Pain 2019; (Epub) B14 ameliorates bone cancer pain through down-regulating spinal IL-1β via suppressing neuron JAK2/STAT3 pathway.
Reactivity : Rat
Zhao Z et al. J Neuroimmune Pharmacol 2019; Neuroprotective Effects of Genistein in a SOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis.
Reactivity : Mouse
Yang CH et al. J Virol 2019; 93 (11) A Novel Murine Model Expressing a Chimeric mSCARB2/hSCARB2 Receptor Is Highly Susceptible to Oral Infection with Clinical Isolates of Enterovirus 71.
Application : IHC-P
Reactivity : Human
Wang SW et al. Alzheimers Res Ther 2017; 9 (1):41 A vaccine with Aβ oligomer-specific mimotope attenuates cognitive deficits and brain pathologies in transgenic mice with Alzheimer's disease.
Application : IHC-P
Reactivity : Mouse
Yang X et al. Mol Med Rep 2017; (Epub) Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia.
Application : IHC-P
Reactivity : Rat
Liou AT et al. Sci Rep. 2016; 6 A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71.
Application : WB, IHC
Reactivity : Mouse
Yang S et al. ACS Chem Neurosci 2016; 7 (4):505-18 A Novel Multifunctional Compound Camellikaempferoside B Decreases Aβ Production, Interferes with Aβ Aggregation, and Prohibits Aβ-Mediated Neurotoxicity and Neuroinflammation.
Application : WB, ICC/IF
Kuan YH et al. Neurobiol Dis 2015; 78:134-45 Targeting P(2)X(7) receptor for the treatment of central post-stroke pain in a rodent model.
Application : ICC/IF
Reactivity : Rat
Liao CC et al. J Virol 2014; Immunodeficient Mouse Models with Different Disease Profiles by in vivo Infection with the Same Clinical Isolate of Enterovirus 71.
Application : IHC-P
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169