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Influenza A virus PA protein antibody

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

Cat No. GTX118991

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Influenza virus- infected H1299 lysates , Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates , *influenza virus (A/WSN/33) infected MDCK cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to C-terminal region of Influenza A virus PA (A/WSN/1933(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

Sample (50 µg of whole cell lysate)
A: H1299
B: H1299 infected with influenza virus (MOI=2 h.p.i=8)
GTX118991 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991

GTX118991 ICC/IF Image

Influenza A Virus PA antibody detects polymerase PA (influenza A H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: polymerase PA (influenza A H1N1) protein stained by Influenza A Virus PA antibody (GTX118991) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37°C, 30 min.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

Sample (60 µg of whole cell lysate)
A: DF-1(WSN infected cell lysates)
B: DF-1 cell lysates
GTX118991 diluted at 1:250
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (GTX118991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Influenza A virus PA protein antibody Cat No. GTX118991

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:5000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf