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Integrin beta 3 antibody [N2C1], Internal

Anti-Integrin beta 3 antibody [N2C1], Internal used in Western Blot (WB). GTX111672

Cat No. GTX111672

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HUVEC , HUVEC treat with Sphingosine 1-phosphate

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Integrin beta 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Integrin Subunit Beta 3,Bdplt16,Bdplt2,Cd61,Gp3A,Gpiiia,Gt,Itgb3

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Integrin beta 3 antibody [N2C1], Internal used in Western Blot (WB). GTX111672

GTX111672 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX111672 diluted at 1:1000

REFERENCE
REVIEW

Integrin beta 3 antibody [N2C1], Internal Cat No. GTX111672

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 16-Feb-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549, HeLa, 293T
Amount used : 30 μg
Blocking : 2% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Lane Description : Lane 1: A549, Lane 2: HeLa, Lane 3: 293T
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169