GeneTex
United States (US)

Involucrin antibody [SY8]

Cat No. GTX40202

Host Mouse
Clonality Monoclonal
Clone Name SY8
Isotype IgG1
Application WB, IHC-P, IP
Reactivity Human, Dog, Pig, Monkey, Gorilla, Owl
APPLICATION

Application Note

IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

68 kDa. ( Note )

Specificity/Sensitivity

Keratinocytes of epidermis and other stratified squamous epithelia
PROPERTIES

Form

Liquid

Buffer

Preservative: None. Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Pure involucrin from human keratinocytes.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Involucrin,Ivl

Cellular Localization

Cytoplasmic

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

Database

Research Area

Package List Price ($)
$ 319