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JNK (phospho Thr183) antibody

Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX34387
Anti-JNK (phospho Thr183) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34387
Anti-JNK (phospho Thr183) antibody used in IHC (Paraffin sections) (IHC-P). GTX34387

Cat. No. GTX34387

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Product Note

This antibody is raised against JNK1/2 phosphorylated at Thr183. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221.

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of JNK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 8 , mitogen-activated protein kinase 9 , mitogen-activated protein kinase 10 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK , JNK3 , JNK3A , PRKM10 , SAPK1b , p493F12 , p54bSAPK

Cellular Localization

Cytoplasm,Nucleus,Membrane,Mitochondrion

Database

Research Area

DATA IMAGES

Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX34387

GTX34387 WB Image

WB analysis of PMA-treated MCF7 (A), UV-treated NIH3T3 (B), PMA-treated PC12 (C) whole cell lysates using GTX34387 JNK (phospho Thr183) antibody.

Anti-JNK (phospho Thr183) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34387

GTX34387 ICC/IF Image

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX34387 JNK (phospho Thr183) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-JNK (phospho Thr183) antibody used in IHC (Paraffin sections) (IHC-P). GTX34387

GTX34387 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX34387 JNK (phospho Thr183) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399