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KCNN4 antibody [6C1]

Overexpression
Anti-KCNN4 antibody [6C1] used in Flow cytometry (FACS). GTX00627
Anti-KCNN4 antibody [6C1] used in Western Blot (WB). GTX00627
Anti-KCNN4 antibody [6C1] used in Western Blot (WB). GTX00627
Anti-KCNN4 antibody [6C1] used in Flow cytometry (FACS). GTX00627
Anti-KCNN4 antibody [6C1] used in Live cell imaging (LCI). GTX00627

Cat. No. GTX00627

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6C1

Isotype

 IgM

Application

 WB, ICC/IF, FACS, IP, IHC, LCI

Reactivity

 Human, Mouse, Rat
Package
50 μg ($619)
See all KCNN4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
IHC Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4 (Accession O15554).

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

potassium calcium-activated channel subfamily N member 4 , DHS2 , IK , IK1 , IKCA1 , KCA4 , KCa3.1 , SK4 , hIKCa1 , hKCa4 , hSK4

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KCNN4 antibody [6C1] used in Flow cytometry (FACS). GTX00627

GTX00627 FACS Image

FACS analysis of THP-1 cells using GTX00627 KCNN4 antibody [6C1].
Black : Negative control
Gray : Isotype control
Green : Primary antibody
Dilution : 1:20

Anti-KCNN4 antibody [6C1] used in Western Blot (WB). GTX00627

GTX00627 WB Image

Overexpression

WB analysis of HEK cells transfected with human KCNN4 using GTX00627 KCNN4 antibody [6C1].
Lane 1 : HEK cells transfected with control vector
Lane 2 : HEK cells transfected with human KCa3.1
Dilution : 1:250

Anti-KCNN4 antibody [6C1] used in Western Blot (WB). GTX00627

GTX00627 WB Image

WB analysis of various samples using GTX00627 KCNN4 antibody [6C1].
Lane 1 : MS1 cell lysate
Lane 2 : IEC-6 cell lysate
Lane 3 : LN-CaP cell lysate
Lane 4 : THP-1 cell lysate
Dilution : 1:250

Anti-KCNN4 antibody [6C1] used in Flow cytometry (FACS). GTX00627

GTX00627 FACS Image

FACS analysis of Raji cells using GTX00627 KCNN4 antibody [6C1].
Black : Negative control
Gray : Isotype control
Green : Primary antibody
Dilution : 1:20

Anti-KCNN4 antibody [6C1] used in Live cell imaging (LCI). GTX00627

GTX00627 LCI Image

Live cell imaging analysis of LN-CaP cells using GTX00627 KCNN4 antibody [6C1].
Panel A : Primary antibody
Panel B : Live view of the cells
Panel C : Merged of panel A and B
Dilution : 1:20

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 619