GeneTex
  • Country / Location Selection

United States (US)

Kinectin 1 antibody [C2C3], C-term

Anti-Kinectin 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX112532
Anti-Kinectin 1 antibody [C2C3], C-term used in Western Blot (WB). GTX112532
Anti-Kinectin 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX112532
Anti-Kinectin 1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112532

Cat. No. GTX112532

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Kinectin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037328

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinectin 1 , CG1 , KNT , MU-RMS-40.19

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein

Background

Various cellular organelles and vesicles are transported along the microtubules in the cytoplasm. Likewise, membrane recycling of the endoplasmic reticulum (ER), Golgi assembly at the microtubule organizing center, and alignment of lysosomes along microtubules are all related processes. The transport of organelles requires a special class of microtubule-associated proteins (MAPs). One of these is the molecular motor kinesin (see MIM 148760 and MIM 600025), an ATPase that moves vesicles unidirectionally toward the plus end of the microtubule. Another such MAP is kinectin, a large integral ER membrane protein. Antibodies directed against kinectin have been shown to inhibit its binding to kinesin.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Kinectin 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX112532

GTX112532 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Kinectin 1A(GTX112532) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Kinectin 1 antibody [C2C3], C-term used in Western Blot (WB). GTX112532

GTX112532 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
GTX112532 diluted at 1:1000

Anti-Kinectin 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX112532

GTX112532 IHC-P Image

Kinectin 1A antibody [C2C3], C-term detects Kinectin 1A protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Kinectin 1A antibody [C2C3], C-term (GTX112532) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Kinectin 1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112532

GTX112532 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Kinectin 1A(GTX112532) antibody at 1:200 dilution.

REFERENCE

There are currently no references for Kinectin 1 antibody [C2C3], C-term (GTX112532). Be the first to share your publications with this product.

REVIEW

Kinectin 1 antibody [C2C3], C-term Cat. No. GTX112532

Rating ( Average 3 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 22-Sep-2014
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 1% BSA, 25°C, 0.5Hr
Fixation : Paraformaldehyde
Permeabilization /Antigen retrieval : 0.2% Triton X-100
Primary Antibody : 1:50, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169