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LAT (phospho Tyr132) antibody

Orthogonal
Anti-LAT (phospho Tyr132) antibody used in Western Blot (WB). GTX24476
Anti-LAT (phospho Tyr132) antibody used in Western Blot (WB). GTX24476

Cat. No. GTX24476

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all LAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-1 μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human LAT that contains tyrosine 132.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_380381

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

linker for activation of T cells , IMD52 , LAT1 , pp36

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LAT (phospho Tyr132) antibody used in Western Blot (WB). GTX24476

GTX24476 WB Image

Orthogonal

WB analysis of Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5) using GTX24476 LAT (phospho Tyr132) antibody.

Anti-LAT (phospho Tyr132) antibody used in Western Blot (WB). GTX24476

GTX24476 WB Image

Orthogonal

The data was published in the journal Front Immunol in 2015.PMID: 25870599

REFERENCE

Application Reference

Matalon O et al. Sci. Signal. 2016; (Epub) Dephosphorylation of the adaptor LAT and phospholipase C–γ by SHP-1 inhibits natural killer cell cytotoxicity.
Application : WB, ICC/IF
Reactivity : Human
Bilal MY et al. Front Immunol 2015; 6 : 141 GRB2 Nucleates T Cell Receptor-Mediated LAT Clusters That Control PLC-γ1 Activation and Cytokine Production.
Application : WB
Reactivity : Human
Shiheido H et al. Eur J Immunol 2014; Novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLCγ1 following T-cell stimulation.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399