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LC3A antibody [166AT1234]

Orthogonal Overexpression
Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

Cat. No. GTX17380

Reference 16

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 166AT1234

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
200 μl ($399)
See all LC3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:25-200
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This LC3A antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Purification

Protein G purified, followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_2887560

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380

GTX17380 ICC/IF Image

Orthogonal

ICC/IF analysis of U251 cells untreatd (left) or treated with Chloroquine (50 μM,16h) (right) using GTX17380 LC3A antibody [166AT1234].
Green : LC3A
Blue : DAPI
Dilution : 1:25

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

WB analysis of various samples using GTX17380 LC3A antibody [166AT1234].
Lane 1: human brain lysates
Lane 2: mouse brain lysates
Lane 3: rat brain lysates
Dilution : 1:2000

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Overexpression

WB analysis of multiple cell lysate using GTX17380 LC3A antibody [166AT1234].
Lane 1: Y79 (soluble fraction of cell extract)
Lane 2: 293 transfected with human LC3A (whole cell extract)
Dilution : 1:1000

Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380

GTX17380 ICC/IF Image

ICC/IF analysis of U251 cells treated with Chloroquine (50 μM,16h) using GTX17380 LC3A antibody [166AT1234].
Green : LC3A
Blue : cell nuclear
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.2%, 30 min)
Dilution : 1:200

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

The data was published in the Cancer Med in 2020. PMID: 32324343

REFERENCE

Application Reference

Lopez-Toledo G et al. Front Aging Neurosci 2022; 14 : 921573 Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.
Application : WB
Reactivity : Human
Jing Z et al. Cancer Med 2020; 9 (12) : 4324-4338 SNGH16 regulates cell autophagy to promote Sorafenib Resistance through suppressing miR-23b-3p via sponging EGR1 in hepatocellular carcinoma.
Schultz ML et al. Nat Commun 2018; 9 (1) : 3671 Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.
Shih CH et al. Part Fibre Toxicol 2018; 15 (1) : 44 Chronic pulmonary exposure to traffic-related fine particulate matter causes brain impairment in adult rats.
Application : IHC
Reactivity : Rat

Clone Reference

He Z et al. Mol Med Rep 2015; 11 (6):914416 * induces autophagic cell death in prostate cancer cells in vitro.
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REVIEW

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Application Reference
Lopez-Toledo G et al. Front Aging Neurosci 2022; 14:921573 Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.
Application : WB
Reactivity : Human
Jing Z et al. Cancer Med 2020; 9 (12):4324-4338 SNGH16 regulates cell autophagy to promote Sorafenib Resistance through suppressing miR-23b-3p via sponging EGR1 in hepatocellular carcinoma.
Schultz ML et al. Nat Commun 2018; 9 (1):3671 Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.
Shih CH et al. Part Fibre Toxicol 2018; 15 (1):44 Chronic pulmonary exposure to traffic-related fine particulate matter causes brain impairment in adult rats.
Application : IHC
Reactivity : Rat
Clone Reference
Rabionet M et al. Hum Mol Genet. 2015; 24 (17):4792-808 Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
Vlahava VM et al. Cell Signal 2015; 27 (6):1253-63 CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism.
Hsin IL et al. Mol Pharm 2015; 12 (5):1534-43 gmi, an immunomodulatory protein from ganoderma microsporum, potentiates *-induced apoptosis via autophagy in lung cancer cells.
Reactivity : Human
He Z et al. Mol Med Rep 2015; 11 (6):914416 * induces autophagic cell death in prostate cancer cells in vitro.
Islam MA et al. Biomaterials 2014; 35 (5):1584-96 The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice.
Reactivity : Mouse
Zhang Y et al. Blood 2012; 119 (12):2895-905 Induction of autophagy is essential for monocyte-macrophage differentiation.
Application : WB
Reactivity : Human
Zhou D et al. J Infect Dis 2012; 205 (8):1258-67 production of * α by human immunodeficiency virus type 1 in human plasmacytoid dendritic cells is dependent on induction of autophagy.
Application : WB
Reactivity : Human
Xing S et al. Autophagy 2012; 8 (1):63-76 Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction.
Application : WB
Reactivity : Human
Song D et al. J Biol Chem 2011; 286 (18):15797-805 Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis.
Application : WB
Reactivity : Mouse
Tuffy LP et al. Mol Cell Biol 2010; 30 (23):5484-501 Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition.
Application : WB
Reactivity : Mouse
Rouschop KM et al. J Clin Invest 2010; 120 (1):127-41 The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
Application : WB
Reactivity : Human
Byun JY et al. Carcinogenesis 2009; 30 (11):1880-8 The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399