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LC3A antibody

Anti-LC3A antibody used in Western Blot (WB). GTX31934
Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX31934

Cat. No. GTX31934

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all LC3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918)

Product Note

Multiple isoforms are known to exist. This antibody is predicted to detect LC3A, LC3B, and LC3C.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LC3A antibody was raised against a 12 amino acid peptide near the center of human LC3AA.The immunogen is located within amino acids 20 - 70 of LC3A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 alpha , ATG8E , LC3 , LC3A , MAP1ALC3 , MAP1BLC3

Cellular Localization

Cytoplasm, cytoskeleton

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-LC3A antibody used in Western Blot (WB). GTX31934

GTX31934 WB Image

WB analysis of human brain tissue lysate using GTX31934 LC3A antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX31934

GTX31934 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31934 LC3A antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399