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LC3A antibody

Anti-LC3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50635
Anti-LC3A antibody used in Western Blot (WB). GTX50635

Cat. No. GTX50635

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all LC3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.5~10 (R-P-F-K-Q) derived from human LC3A.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174462

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 alpha , ATG8E , LC3 , LC3A , MAP1ALC3 , MAP1BLC3

Cellular Localization

Cytoplasm, cytoskeleton

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-LC3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50635

GTX50635 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50635 LC3A antibody.

Anti-LC3A antibody used in Western Blot (WB). GTX50635

GTX50635 WB Image

WB analysis of extracts from HeLa and 293 cells using GTX50635 LC3A antibody.

REFERENCE

Application Reference

Xing J et al. Nat Immunol 2016; (Epub) Identification of a role for TRIM29 in the control of innate immunity in the respiratory tract.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399