GeneTex
United States (US)

LDHA antibody

GTX101416_40478_20150410_IFA_19011506_634.jpg
GTX101416_40478_IHC_19011506_273.jpg
GTX101416_40478_WB_R_19011506_173.jpg
GTX101416_40478_20151029_WB_19011506_197.jpg
GTX101416_40478_WB_M_19011506_774.jpg
GTX101416_42669_20161117_WB_19011506_524.jpg
GTX101416_40478_20150410_IFA_2_19011506_387.jpg
GTX101416_40478_IFA_19011506_392.jpg

Cat No. GTX101416

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , MCF-7 , MDA-MB-231 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lactate Dehydrogenase A,Gsd11,Hel-S-133P,Ldhm,Pig19,Ldha

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101416_40478_20150410_IFA_19011506_634.jpg

GTX101416 ICC/IF Image

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101416_40478_IHC_19011506_273.jpg

GTX101416 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using LDHA(GTX101416) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101416_40478_WB_R_19011506_173.jpg

GTX101416 WB Image

LDHA antibody detects LDHA protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (GTX101416) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101416_40478_20151029_WB_19011506_197.jpg

GTX101416 WB Image

LDHA antibody detects LDHA protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody (GTX101416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101416_40478_WB_M_19011506_774.jpg

GTX101416 WB Image

LDHA antibody detects LDHA protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
E. 30 µg C2C12 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (GTX101416) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101416_42669_20161117_WB_19011506_524.jpg

GTX101416 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with LDHA antibody (GTX101416) diluted at 1:1000.

GTX101416_40478_20150410_IFA_2_19011506_387.jpg

GTX101416 ICC/IF Image

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101416_40478_IFA_19011506_392.jpg

GTX101416 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LDHA(GTX101416) antibody at 1:500 dilution.

REFERENCE
REVIEW

LDHA antibody Cat No. GTX101416

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Blocking : 5%milk in TBST
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf