APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89916-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence TGSVDQTVKVWECR, from the C Terminus of the protein sequence according to NP_000421.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
platelet activating factor acetylhydrolase 1b regulatory subunit 1 , LIS1 , LIS2 , MDCR , MDS , NudF , PAFAH
Cellular Localization
Cytoplasm, cytoskeleton
Background
This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq, Apr 2009]
Database
Research Area
DATA IMAGES
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GTX89916 IHC-P Image
IHC-P analysis of human pancreas using GTX89916 LIS1 antibody, C-term.
Antigen retrieval : citrate buffer Ph 6
Dilution : 3.75μg/ml
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GTX89916 WB Image
WB analysis of rat ovary lysate using GTX89916 LIS1 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89916 IHC-P Image
IHC-P analysis of human cortex using GTX89916 LIS1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89916 IHC-P Image
IHC-P analysis of human thyroid using GTX89916 LIS1 antibody, C-term.
Antigen retrieval : citrate buffer Ph 6
Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for LIS1 antibody, C-term (GTX89916). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for LIS1 antibody, C-term (GTX89916). Be the first to share your experience with this product.
