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LRG1 antibody

Anti-LRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117875
Anti-LRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117875
Anti-LRG1 antibody used in Western Blot (WB). GTX117875
Anti-LRG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117875

Cat. No. GTX117875

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LRG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

human plasma

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618478

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich alpha-2-glycoprotein 1 , HMFT1766 , LRG

Cellular Localization

Secreted

Background

The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117875

GTX117875 IHC-P Image

LRG1 antibody detects LRG1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LRG1 antibody (GTX117875) dilution: 1:250.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117875

GTX117875 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using LRG1(GTX117875) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LRG1 antibody used in Western Blot (WB). GTX117875

GTX117875 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LRG1 antibody (GTX117875) diluted at 1:1000.

Anti-LRG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117875

GTX117875 ICC/IF Image

LRG1 antibody detects LRG1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LRG1 protein stained by LRG1 antibody (GTX117875) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

LRG1 antibody Cat. No. GTX117875

Rating
( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-LRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117875
Date : Anonymous submitted on 02-Dec-2013
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : breast carcinoma tissue
Fixation : 10% formalin
Permeabilization /Antigen retrieval : CC1
Primary Antibody : 1:250, 37°C, 1Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169