GeneTex

  • Country / Location Selection

United States (US)

LRP130 antibody [N2N3]

KO/KD
Anti-LRP130 antibody [N2N3] used in Western Blot (WB). GTX115351
Anti-LRP130 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX115351
Anti-LRP130 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115351
Anti-LRP130 antibody [N2N3] used in Western Blot (WB). GTX115351

Cat. No. GTX115351

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LRP130 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

Positive Control

HepG2 , HCT116 , 293T

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LRP130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719753

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich pentatricopeptide repeat containing , CLONE-23970 , GP130 , LRP130 , LSFC

Cellular Localization

Mitochondrion , Nucleus , nucleoplasm , Nucleus inner membrane , Nucleus outer membrane

Background

This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LRP130 antibody [N2N3] used in Western Blot (WB). GTX115351

GTX115351 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N2N3] (GTX115351) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LRP130 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX115351

GTX115351 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using LRP130(GTX115351) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LRP130 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115351

GTX115351 ICC/IF Image

LRP130 antibody [N2N3] detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: LRP130 protein stained by LRP130 antibody [N2N3] (GTX115351) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-LRP130 antibody [N2N3] used in Western Blot (WB). GTX115351

GTX115351 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
5% SDS PAGE
GTX115351 diluted at 1:1000

REFERENCE

There are currently no references for LRP130 antibody [N2N3] (GTX115351). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for LRP130 antibody [N2N3] (GTX115351). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169