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Lck (phospho Tyr192) antibody

Orthogonal
Anti-Lck (phospho Tyr192) antibody used in Western Blot (WB). GTX24900

Cat. No. GTX24900

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μl ($399)
See all Lck products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the regionof human Lck that contains tyrosine 192 (based on Swiss Protein database, accession number P06239).The sequence is conserved in mouse.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LCK proto-oncogene, Src family tyrosine kinase , IMD22 , LSK , YT16 , p56lck , pp58lck

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-Lck (phospho Tyr192) antibody used in Western Blot (WB). GTX24900

GTX24900 WB Image

Orthogonal

WB (peptide competition) analysis of background extracts alone (Lane 1) or with added recombinant active human Lck protein (Lane 2-5) using GTX24900 Lck (phospho Tyr192) antibody prior incubated with the non-phosphopeptide corresponding to the non-phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4) or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399