GeneTex
United States (US)

Leptin recombinant protein

Leptin-recombinant-protein_WB_GTX65363-1_18121411_403.jpg

Cat No. GTX65363

Application WB, ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

Protein's bioactivity determined in an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 mg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

Calculated MW

16.2 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute in 10 mM HCl to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Obesity Protein, OBS, OB Protein, Obesity factor., LEP

Background

Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Murine Leptin is a 16 kDa protein containing 147 amino acid residues.

Research Area

DATA IMAGES
Leptin-recombinant-protein_WB_GTX65363-1_18121411_403.jpg

GTX65363 WB Image

Western blot analysis of leptin using GTX65363

Package List Price ($)
$ 199