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Ly6g antibody [RB6-8C5]

Cat. No. GTX40912

Reference 13

Host

 Rat

Clonality

 Monoclonal

Clone Name

 RB6-8C5

Isotype

 IgG2b

Application

 IHC-P, IHC-Fr, FACS, Depletion

Reactivity

 Human, Mouse, Rat
Package
100 μg ($369)
See all Ly6g products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Depletion Assay dependent

Note :

Depletion
in vivo depletion of Gr-1+ myeloid cells
Not tested in other applications.

Product Note

The RB6-8C5 monoclonal antibody reacts strongly with mouse Ly6G and weakly with mouse Ly6C previously referred to as GR-1. The RB6-8C5 antibody has been shown to inhibit the binding of the 1A8 antibody. The 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse granulocytes

Purification

Protein G purified
From tissue culture supernatant

Purity

>95% (Determined by SDS-PAGE)

Endotoxin

< 0.002 EU/弮g (Determined by LAL assay)

Conjugation

Unconjugated

RRID

AB_11167377

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Gr1,Ly6G,Ly6g,lymphocyte antigen 6 complex, locus G,Gr-1,Ly-6G

Database

Research Area

REFERENCE

Application Reference

Wang W et al. Cell Transplant 2024; 33 : 9636897241236584 Mechanical Unloading Promotes Osteoclastic Differentiation and Bone Resorption by Modulating the MSC Secretome to Favor Inflammation.
Yuanjia Zhu et al. Sci Rep 2022; 12 (1) : 10028 A novel photosynthetic biologic topical gel for enhanced localized hyperoxygenation augments wound healing in peripheral artery disease.
Application : IHC-Fr
Reactivity : Rat
Xu Q et al. Front Pharmacol 2021; 12 : 655372 Emodin Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury by Inhibiting the Cold-Inducible RNA-Binding Protein (CIRP)-Mediated Activation of the NLRP3/IL-1β/CXCL1 Signaling.

Clone Reference

Yamamoto Y et al. Life Sci Alliance 2022; 5 (10) Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.
Manriquez V et al. et al. Nat Commun 2021; 12 (1):4547 Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils
Application : IHC-Fr
Reactivity : Human
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REVIEW

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Application Reference
Wang W et al. Cell Transplant 2024; 33:9636897241236584 Mechanical Unloading Promotes Osteoclastic Differentiation and Bone Resorption by Modulating the MSC Secretome to Favor Inflammation.
Yuanjia Zhu et al. Sci Rep 2022; 12 (1):10028 A novel photosynthetic biologic topical gel for enhanced localized hyperoxygenation augments wound healing in peripheral artery disease.
Application : IHC-Fr
Reactivity : Rat
Xu Q et al. Front Pharmacol 2021; 12:655372 Emodin Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury by Inhibiting the Cold-Inducible RNA-Binding Protein (CIRP)-Mediated Activation of the NLRP3/IL-1β/CXCL1 Signaling.
Liang H et al. PeerJ 2021; 9:e10644 Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats.
Application : IHC-P
Reactivity : Rat
Wu F et al. Inflammation 2019; (Epub) Cellular Inflammatory Response of the Spleen After Acute Spinal Cord Injury in Rat.
Application : IHC-P
Reactivity : Rat
Calcinotto A et al. Nature 2018; 559 (7714):363-369 IL-23 secreted by myeloid cells drives castration-resistant prostate cancer.
Application : IHC-P
Reactivity : Mouse
Muller I et al. Circ Heart Fail 2017; 10 (11) Pathogenic Role of the Damage-Associated Molecular Patterns S100A8 and S100A9 in Coxsackievirus B3-Induced Myocarditis.
Reactivity : Mouse
Muller I et al. PLoS One 2017; 12 (8):e0182643 CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis.
Application : IHC-Fr
Clone Reference
Yamamoto Y et al. Life Sci Alliance 2022; 5 (10) Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.
Manriquez V et al. et al. Nat Commun 2021; 12 (1):4547 Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils
Application : IHC-Fr
Reactivity : Human
Pollenus E et al. Immunol Lett. 2019; 212:30-36 Limitations of neutrophil depletion by anti-Ly6G antibodies in two heterogenic immunological models.
Application : FACS
Reactivity : Mouse
Xu D et al. Front Cell Infect Microbiol. 2018; 8:157 Gr-1+ Cells Other Than Ly6G+ Neutrophils Limit Virus Replication and Promote Myocardial Inflammation and Fibrosis Following Coxsackievirus B3 Infection of Mice.
Application : FACS
Reactivity : Mouse
Jinjin Xu et al. Nat Commun. 2016; The REGγ-proteasome forms a regulatory circuit with IκB? and NFκB in experimental colitis.
Application : IHC-P, FACS
Reactivity : Mouse
Package List Price ($)
$ 369