The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells is <5.0 ng/ml, corresponding to a specific activity of ≥2 x10^5 units/mg.
Sterile filtered and then lyophilized from 5 mM Tris, pH 8.0 + 80 mM Arginine.
Storage
Reconstitute in water to a concentration of 0.1-0.5 mg/ml. The solution can then be diluted into other aqueous buffers. Stored at -20ºC for future use.
Antigen Species
Human
Expression System
E. coli
Purification
Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Colony Stimulating Factor 1,Csf-1,Mcsf,Csf1
Background
Macrophage Colony stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, microphages, and endothelial cells. M-CSF is involved in both the development and function of monocytes/microphages. Recombinant human M-CSF is a 36.8 kDa homodimeric protein containing two 159 amino acid polypeptide subunits.