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MAP2 antibody [GT925]

Anti-MAP2 antibody [GT925] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634473
Anti-MAP2 antibody [GT925] used in Western Blot (WB). GTX634473
Anti-MAP2 antibody [GT925] used in IHC (Paraffin sections) (IHC-P). GTX634473

Cat No. GTX634473

Host Mouse
Clonality Monoclonal
Clone Name GT925
Isotype IgG3
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

199 kDa. ( Note )

Positive Control

mouse brain , rat brain , DIV10 rat E18 primary cortical neuron
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

microtubule-associated protein 2,G1-397-34,MAP-2,Mtap-2,Mtap2,repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Database

Research Area

DATA IMAGES
Anti-MAP2 antibody [GT925] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634473

GTX634473 ICC/IF Image

MAP2 antibody [GT925] detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dendrite marker MAP2 stained by MAP2 antibody [GT925] (GTX634473) diluted at 1:500.
Red: Axon marker Tau, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody [GT925] used in Western Blot (WB). GTX634473

GTX634473 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody [GT925] (GTX634473) diluted at 1:6000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MAP2 antibody [GT925] used in IHC (Paraffin sections) (IHC-P). GTX634473

GTX634473 IHC-P Image

MAP2 antibody [GT925] detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
MAP2 stained by MAP2 antibody [GT925] (GTX634473) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169