MAP2 antibody

Cat. No. GTX82661

Reference 6

Host	Chicken
Clonality	Polyclonal
Isotype	IgY
Application	WB, ICC/IF, IHC, PLA
Reactivity	Human, Mouse, Rat, Bovine
Package	100 μl ($439)

See all MAP2 products

View product citations for antibody GTX82661 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:20000
ICC/IF	1:1000-1:20000
IHC	1:2500-1:10000
PLA	Assay dependent

Not tested in other applications.

Calculated MW

200 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

10mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Recombinant bovine MAP2 protein expressed in and purified from E. Coli.

Purification

Total IgY fraction

Conjugation

Unconjugated

RRID

AB_11172558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 2 , MAP-2 , MAP2A , MAP2B , MAP2C

Cellular Localization

Cytoplasm, cytoskeleton , Cell projection, dendrite

Background

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Database

Gene ID: 4133 MAP2
UniProt: P11137 MAP2

Research Area

DATA IMAGES

GTX82661 ICC/IF Image

ICC/IF analysis of neuron-glial cell cultures from E20 rat using GTX82661 MAP2 antibody. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Red : MAP2
Green : Tau
Blue : DAPI
Dilution : 1:10,000

GTX82661 WB Image

WB analysis of rat cortical lysate using GTX82661 MAP2 antibody.

GTX82661 IHC Image

IHC analysis of rat brain stem tissue using GTX82661 MAP2 antibody. The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons.
Red : MAP2
Green : FOX3
Dlue : DAPI
Dilution : 1:5000