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MAPK15 antibody

Anti-MAPK15 antibody used in Western Blot (WB). GTX107519
Anti-MAPK15 antibody used in IHC (Paraffin sections) (IHC-P). GTX107519
Anti-MAPK15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107519

Cat. No. GTX107519

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAPK15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HeLaS3

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAPK15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950874

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 15 , ERK7 , ERK8

Background

In vitro, phosphorylates MBP.

Database

Research Area

DATA IMAGES

Anti-MAPK15 antibody used in Western Blot (WB). GTX107519

GTX107519 WB Image

Sample
A: 30 μg of HeLa S3 whole cell lysate (GTX14654)
7.5% SDS PAGE
GTX107519 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAPK15 antibody used in IHC (Paraffin sections) (IHC-P). GTX107519

GTX107519 IHC-P Image

MAPK15 antibody detects MAPK15 protein at cytosol on human lung by immunohistochemical analysis.
Sample: Paraffin-embedded lung.
MAPK15 antibody (GTX107519) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAPK15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107519

GTX107519 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MAPK15(GTX107519) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169