GeneTex
United States (US)

MAPKAPK-2 (Active) recombinant protein

Cat No. GTX65303

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

254 nmol phosphate incorporated into MAPKAP kinase 2 substrate peptide per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP and total of 0.83 uCi/ul P-32.

Calculated MW

68.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥90%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MAP kinaseactivated protein kinase2, MAP kinase-activated protein kinase 2, MAP kinaseactivated protein kinase 2, MAPKAPK2, MAPKAPK, MK2

Background

MAPKAP kinase2 is a Ser/Thr protein kinase, which is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo.
Package List Price ($)
$ 359