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MARCH8 antibody

Anti-MARCH8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85022
Anti-MARCH8 antibody used in Western Blot (WB). GTX85022

Cat. No. GTX85022

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all MARCH8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8.The immunogen is located within the last 50 amino acids of MARCH8.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane associated ring-CH-type finger 8 , CMIR , MARCH-VIII , MIR , RNF178 , c-MIR

Cellular Localization

Lysosome membrane

Background

MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Database

Research Area

DATA IMAGES

Anti-MARCH8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85022

GTX85022 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85022 MARCH8 antibody.
Working concentration : 2.5 μg/ml

Anti-MARCH8 antibody used in Western Blot (WB). GTX85022

GTX85022 WB Image

WB analysis of HeLa cell lysate using GTX85022 MARCH8 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409