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MARCKS (phospho Ser170) antibody

Anti-MARCKS (phospho Ser170) antibody used in Western Blot (WB). GTX50348
Anti-MARCKS (phospho Ser170) antibody used in IHC (Paraffin sections) (IHC-P). GTX50348
Anti-MARCKS (phospho Ser170) antibody used in Western Blot (WB). GTX50348

Cat. No. GTX50348

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MARCKS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 170 (G-F-S(p)-F-K) derived from human MARCKS.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myristoylated alanine rich protein kinase C substrate , 80K-L , MACS , PKCSL , PRKCSL

Cellular Localization

Cytoplasm, cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MARCKS (phospho Ser170) antibody used in Western Blot (WB). GTX50348

GTX50348 WB Image

WB analysis of extracts from 3T3 cells treated with serum or calf intestinal phosphatase (CIP) using GTX50348 MARCKS (phospho Ser170) antibody.

Anti-MARCKS (phospho Ser170) antibody used in IHC (Paraffin sections) (IHC-P). GTX50348

GTX50348 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50348 MARCKS (phospho Ser170) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-MARCKS (phospho Ser170) antibody used in Western Blot (WB). GTX50348

GTX50348 WB Image

WB analysis of extracts from MCF cells untreated or treated with EGF using GTX50348 MARCKS (phospho Ser170) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399