APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
3xFlag-human MBD4-transfected 293T cells
Predict Reactivity
Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MBD4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886051
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
methyl-CpG binding domain 4, DNA glycosylase , MED1
Cellular Localization
Nucleus
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129605 WB Image
MBD4 antibody detects MBD4 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human MBD4-transfected 293T cells
7.5 % SDS-PAGE
MBD4 antibody (GTX129605) dilution: 1:5000
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GTX129605 ICC/IF Image
MBD4 antibody detects MBD4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MBD4 protein stained by MBD4 antibody (GTX129605) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
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REVIEW
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