The specific activity as determined by the ability of MCP-4 to chemoattract human monocytes was achieved at 10-100 ng/ml.
Sterile filtered and then lyophilized from a concentrated (1mg/ml) sterile solution containing 20 mM phosphate buffer, pH 7.4 and 130 mM NaCl.
Reconstitute in water to a concentration of not less than 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at –20ºC for future use.
Purity was assessed by SDS-PAGE (≥95%) and by HPLC (≥95%).
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Monocyte Chemotactic Protein4, NCC-1, Monocyte Chemotactic Protein-4, CCL13, NCC1, MCP4, NCC 1, Monocyte Chemotactic Protein 4
Human macrophage/monocyte chemotactic protein-4 (MCP-4) is a member of the beta chemokine family (C-C) of cytokines. Like eotaxin, MCP-4 is a powerful eosinophil chemoattractant. Additionally, like MCP-3, MCP-4 elicits chemoattraction of monocytes and T lymphocytes. MCP-4 is a peptide of 8.6 kDa that consists of 75 amino acid residues.