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MED10 antibody

Orthogonal
Anti-MED10 antibody used in Western Blot (WB). GTX131860
Anti-MED10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131860

Cat. No. GTX131860

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MED10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T nuclear extract

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MED10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886530

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator complex subunit 10 , L6 , NUT2 , TRG20

Cellular Localization

Nucleus

Background

MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MED10 antibody used in Western Blot (WB). GTX131860

GTX131860 WB Image

Orthogonal

293T whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 antibody (GTX131860) diluted at 1:500.

Anti-MED10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131860

GTX131860 ICC/IF Image

MED10 antibody detects MED10 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MED10 protein stained by MED10 antibody (GTX131860) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169