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MEK1 + MEK2 antibody

Overexpression
Anti-MEK1 + MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111338
Anti-MEK1 + MEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111338
Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX111338
Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX111338
Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX111338

Cat. No. GTX111338

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MEK1 + MEK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

MEK1-transfected 293T , MEK2-transfected 293T , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK1 + MEK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722030

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 1 , mitogen-activated protein kinase kinase 2 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1 , CFC4 , MAPKK2 , MEK2 , MKK2 , PRKMK2

Database

Research Area

DATA IMAGES

Anti-MEK1 + MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111338

GTX111338 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using MEK1 + MEK2 antibody (GTX111338) at 1:500 dilution.

Anti-MEK1 + MEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111338

GTX111338 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MEK1 + MEK2 antibody (GTX111338) at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX111338

GTX111338 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX111338) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX111338

GTX111338 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX111338) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX111338

GTX111338 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX111338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169