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MEK5 antibody

Anti-MEK5 antibody used in Western Blot (WB). GTX55702
Anti-MEK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55702
Anti-MEK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55702
Anti-MEK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55702
Anti-MEK5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55702

Cat. No. GTX55702

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MEK5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MAP2K5 (NP_660143.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 5 , HsT17454 , MAPKK5 , MEK5 , PRKMK5

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-MEK5 antibody used in Western Blot (WB). GTX55702

GTX55702 WB Image

WB analysis of various sample lysates using GTX55702 MEK5 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-MEK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55702

GTX55702 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX55702 MEK5 antibody.
Dilution : 1:100

Anti-MEK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55702

GTX55702 IHC-P Image

IHC-P analysis of human appendix tissue using GTX55702 MEK5 antibody.
Dilution : 1:100

Anti-MEK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55702

GTX55702 IHC-P Image

IHC-P analysis of rat heart tissue using GTX55702 MEK5 antibody.
Dilution : 1:100

Anti-MEK5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55702

GTX55702 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX55702 MEK5 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399