MERS-CoV Nucleoprotein antibody

Name: MERS-CoV Nucleoprotein antibody
Brand: GeneTex
SKU: GTX134868
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868

Anti-MERS-CoV Nucleoprotein antibody used in ELISA (ELISA). GTX134868

Cat. No. GTX134868

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA
Reactivity	Middle East respiratory syndrome coronavirus
Package	100 μl ($399), 25 μl ($169)

See all MERS-CoV Nucleoprotein products

View product citations for antibody GTX134868 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Product Note

This antibody detects MERS-CoV Nucleoprotein, but does not cross-react with SARS-CoV or SARS-CoV-2 nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of MERS-CoV Nucleoprotein (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887364

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX134868 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (GTX134868) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX134868 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant MERS-CoV Nucleocapsid protein (GTX135653-pro) (88.75-1.39 nM). Coated protein was probed with MERS-CoV Nucleoprotein antibody (GTX134868) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX134868 WB Image

Non-infected and infected Vero B4 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (GTX134868) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Weskamm LM et al. Cell Rep Med 2022; 3 (7) : 100685 Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine.

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