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MERS-CoV Spike (S1) antibody

Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875
Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875

Cat. No. GTX134875

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Middle East respiratory syndrome coronavirus
Package
100 μl ($399),
25 μl ($169)
See all MERS-CoV Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
Not tested in other applications.

Positive Control

MERS-CoV (NCoV / Novel coronavirus) Spike Protein-tagged protein-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Recombinant protein encompassing a sequence within the center region of MERS-CoV Spike (S1) (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887370

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875

GTX134875 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MERS-CoV S1 protein antibody (GTX134875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875

GTX134875 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MERS-CoV S1 protein antibody (GTX134875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169