GeneTex
United States (US)

MHC Class II H-2I-Ak/S antibody [OX-6]

Cat No. GTX39680

Host Mouse
Clonality Monoclonal
Clone Name OX-6
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/50-1/100
IHC-Fr Assay dependent
FACS 1/50-1/100

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Specificity/Sensitivity

GTX39681/GTX39680 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat thymocyte membrane glycoproteins.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
REFERENCE
Package List Price ($)
$ 319