GeneTex
United States (US)

MIF (Active) recombinant protein

Cat No. GTX65173

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Human MIF biological activity was measured in an agarose micro droplet assay using human U937 cells as targets. The optimal range of activity found for human MIF in this assay was 0.5-1ug/ml.

Calculated MW

12.5 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

10 mM sodium phosphate buffer pH-7.5.

Storage

Reconstitute in sterile ddH2O. Reconstitute in ddH2O. Store at -20ºC. Product is stable for at least 12 months.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥97%) and by HPLC (≥97%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glycosylation-inhibiting factor, MMIF, MIF, Glycosylationinhibiting factor, Phenylpyruvate tautomerase, MIF., GIF, GLIF

Background

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12.5 kDa.

Research Area