GeneTex
United States (US)

MMP1 antibody

GTX100534_39918_IHC_19011506_778.jpg
GTX100534_39918_IHC_3_19011506_808.jpg
GTX100534_39918_IHC_M_19011506_265.jpg
GTX100534_39918_IHC_2_19011506_818.jpg
GTX100534_42865_20170622_WB_19011506_435.jpg

Cat No. GTX100534

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Raji , HUVEC , *dermal fibroblast cells

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 1,Clg,Clgn,Mmp1

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100534_39918_IHC_19011506_778.jpg

GTX100534 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MMP1(GTX100534) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100534_39918_IHC_3_19011506_808.jpg

GTX100534 IHC-P Image

MMP1 antibody detects MMP1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP1 antibody (GTX100534) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100534_39918_IHC_M_19011506_265.jpg

GTX100534 IHC-P Image

MMP1 antibody detects MMP1 protein at secreted on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
MMP1 antibody (GTX100534) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100534_39918_IHC_2_19011506_818.jpg

GTX100534 IHC-P Image

MMP1 antibody detects MMP1 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
MMP1 antibody (GTX100534) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100534_42865_20170622_WB_19011506_435.jpg

GTX100534 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP1 antibody (GTX100534) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

MMP1 antibody Cat No. GTX100534

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 20-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : dermal fibroblast cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169