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MMP1 antibody

Orthogonal
Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100534
Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100534
Anti-MMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100534
Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100534
Anti-MMP1 antibody used in Western Blot (WB). GTX100534
Anti-MMP1 antibody used in Western Blot (WB). GTX100534
Anti-MMP1 antibody used in Western Blot (WB). GTX100534

Cat. No. GTX100534

Reference 21
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MMP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950926

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 1 , CLG , CLGN

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100534

GTX100534 IHC-P Image

MMP1 antibody detects MMP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100534

GTX100534 IHC-P Image

MMP1 antibody detects MMP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100534

GTX100534 ICC/IF Image

MMP1 antibody detects MMP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100534

GTX100534 IHC-P Image

MMP1 antibody detects secreted MMP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MMP1 antibody used in Western Blot (WB). GTX100534

GTX100534 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP1 antibody (GTX100534) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP1 antibody used in Western Blot (WB). GTX100534

GTX100534 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2017.PMID: 28489070

Anti-MMP1 antibody used in Western Blot (WB). GTX100534

GTX100534 WB Image

Orthogonal

The data was published in the journal Aging Dis in 2019. PMID: 31164994

REFERENCE

Application Reference

Yan Guo et al. iScience 2022; 25 (7) : 104519 Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration.
Seungje Jeon et al. Aging (Albany NY) 2021; 13 (5) : 6406-6419 Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway.
Application : WB
Reactivity : Mouse
Mandal JP et al. Free Radic Biol Med 2021; 163 : 69-87 PKCδ mediates mitochondrial ROS generation and oxidation of HSP60 to relieve RKIP inhibition on MAPK pathway for HCC progression.
Application : WB
Reactivity : Human
Lee KE et al. Biomolecules 2020; 10 (10) Computational and In Vitro Investigation of (-)-Epicatechin and Proanthocyanidin B2 as Inhibitors of Human Matrix Metalloproteinase 1.
Application : WB
Mahdavi N et al. Indian J Pathol Microbiol 2020; 63 (3) : 369-375 Evaluation of the presence of myofibroblasts and matrix metalloproteinase 1 expression in the stroma of oral verrucous hyperplasia and verrucous carcinoma.
Application : IHC-P
Reactivity : Human
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Submit a Reference

REVIEW

MMP1 antibody Cat. No. GTX100534

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-MMP1 antibody used in Western Blot (WB). GTX100534
Date : Anonymous submitted on 25-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : OSCC-SAS cells with/without chemical treatment
Amount used : 40μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : Undiluted, 4°C, 18Hr
Anti-MMP1 antibody used in Western Blot (WB). GTX100534
Date : Anonymous submitted on 29-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : ASF-4-1 cells
Amount used : 4μg
Blocking : Skim milk, 25°C, 1Hr
Primary Antibody : 1:6000, 4°C, 12Hr
Anti-MMP1 antibody used in Western Blot (WB). GTX100534
Date : Anonymous submitted on 20-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : dermal fibroblast cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Submit a Review
Application Reference
Yan Guo et al. iScience 2022; 25 (7):104519 Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration.
Seungje Jeon et al. Aging (Albany NY) 2021; 13 (5):6406-6419 Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway.
Application : WB
Reactivity : Mouse
Mandal JP et al. Free Radic Biol Med 2021; 163:69-87 PKCδ mediates mitochondrial ROS generation and oxidation of HSP60 to relieve RKIP inhibition on MAPK pathway for HCC progression.
Application : WB
Reactivity : Human
Lee KE et al. Biomolecules 2020; 10 (10) Computational and In Vitro Investigation of (-)-Epicatechin and Proanthocyanidin B2 as Inhibitors of Human Matrix Metalloproteinase 1.
Application : WB
Mahdavi N et al. Indian J Pathol Microbiol 2020; 63 (3):369-375 Evaluation of the presence of myofibroblasts and matrix metalloproteinase 1 expression in the stroma of oral verrucous hyperplasia and verrucous carcinoma.
Application : IHC-P
Reactivity : Human
Wang C et al. Biotechnol Lett 2020; 42 (8):1567-1579 Mechanical injury and IL-1β regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes.
Application : WB
Reactivity : Human
Dubey NK et al. Aging Dis 2019; 10 (3):483-496 Adipose-derived Stem Cells Attenuates Diabetic Osteoarthritis via Inhibition of Glycation-mediated Inflammatory Cascade.
Wu PY et al. Antioxidants (Basel) 2019; 8 (10) 1,2-Bis[(3-Methoxyphenyl)Methyl]Ethane-1,2-Dicarboxylic Acid Reduces UVB-Induced Photodamage In Vitro and In Vivo.
Application : WB
Reactivity : Human
Bordignon P et al. Cell Rep 2019; 28 (9):2358-2372.e6 Dualism of FGF and TGF-帣 Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant.
Application : WB
Reactivity : Human
Liu J et al. Mol Med Rep 2019; 20 (1):33-40 miR?338?3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β?catenin signaling pathways.
Application : ICC/IF
Reactivity : Human
Wei LY et al. Oral Oncol 2019; 88:115-123 Reciprocal activation of cancer-associated fibroblasts and oral squamous carcinoma cells through CXCL1.
Application : WB
Reactivity : Human
Olaya-C M et al. Int J Womens Health 2018; 10:783-795 The umbilical cord, preeclampsia and the VEGF family.
Kumar Dubey Navneet et al. Aging and Disease 2018; (Epub) Adipose-derived Stem Cells Attenuates Diabetic Osteoarthritis via Inhibition of Glycation-mediated Inflammatory Cascade.
Application : WB
Reactivity : Mouse
Rusdamayanti Rusdamayanti et al. Indonesian Journal of Pathology 2018; 27:2 Ekspresi E-cadherin dan MMP-1 pada Adenokarsinoma Kolorek-tal dengan dan Tanpa Metastasis KGB.
Lin KT et al. Am J Chin Med 2018;:43116 Rhodiola crenulata Attenuates γ-Ray Induced Cellular Injury Via Modulation of Oxidative Stress in Human Skin Cells.
Application : WB
Reactivity : Human
Kim YH et al. Nat Commun 2017; 8:15208 Senescent tumor cells lead the collective invasion in thyroid cancer.
Han SH et al. Int J Mol Sci 2017; 18 (6) The Implication of Substance P in the Development of Tendinopathy: A Case Control Study.
Application : WB
Reactivity : Human
Chen WL et al. PLoS One 2015; 10 (9):e0137979 Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Application : WB
Reactivity : Human
Dagistan Y et al. World Neurosurg 2015; 84 (6):1739-46 Importance of IL-6, MMP-1, IGF-1, and BAX Levels in Lumbar Herniated Disks and Posterior Longitudinal Ligament in Patients with Sciatic Pain.
Reactivity : Human
Lin CY et al. J Mol Med (Berl) 2014; A dual tyrosine kinase inhibitor * suppresses overexpression of matrix metallopeptidase 1 (MMP1) in endometrial cancer.
Application : WB
Reactivity : Human
Sandner A et al. Exp Cell Res 2013; 319 (19):2905-15 Reflux induces DNA strand breaks and expression changes of MMP1+9+14 in a human miniorgan culture model.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169