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MTF1 antibody

Anti-MTF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129220

Cat. No. GTX129220

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MTF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MTF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885927

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

metal regulatory transcription factor 1 , MTF-1 , ZRF

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MTF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129220

GTX129220 ICC/IF Image

MTF1 antibody detects MTF1 protein at nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MTF1 protein stained by MTF1 antibody (GTX129220) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169