GeneTex
United States (US)

MVP/LRP antibody [0.N.389]

Cat No. GTX14563

Host Mouse
Clonality Monoclonal
Clone Name 0.N.389
Isotype IgG1
Application WB, IHC-P, FACS, IP, GSA
Reactivity Human, Rat
APPLICATION

Application Note

FACS: Use at an assay dependent dilution. GSA: Use at an assay dependent dilution. IHC-P: Use at a concentration of 1 - 2 μg/ml for 30 min at RT (staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min). IP: Use 2μg/mg protein lysate (Use Protein G). WB: Use at a concentration of 1 - 2 μg/ml for 2 hrs at RT. Predicted molecular weight: 110 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

99 kDa. ( Note )

Positive Control

SKBR3 cells and breast carcinomas
PROPERTIES

Form

Liquid

Buffer

Preservative: none; Constituents: PBS. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Proteins purified from extract of human breast cancer MCF-7 cells.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Major Vault Protein,Lrp,Vault1,Mvp

Cellular Localization

Cytoplasmic, 5% are nucleus associated and localize to the nuclear pore complexes

Background

Major vault protein (MVP) is identical to lung resistance related protein (LRP). Its shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocortoid receptors are also able to co-immunoprecipitate the MVP. Reportedly, the lung resistance related protein (LRP) is identical to human MVP. LRP is overexpressed in many neoplastic tissues and cells lines indicating a poor response to chemotherapy.

Database

Package List Price ($)
$ 329