GeneTex

  • Country / Location Selection

United States (US)

MYF5 antibody

Anti-MYF5 antibody used in Western Blot (WB). GTX87110
Anti-MYF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX87110
Anti-MYF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87110
Anti-MYF5 antibody used in Western Blot (WB). GTX87110

Cat. No. GTX87110

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μg ($399)
See all MYF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MYF5 (21-70).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10724548

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MYF5 , bHLHc2 , myogenic factor 5

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-MYF5 antibody used in Western Blot (WB). GTX87110

GTX87110 WB Image

WB analysis of COS7 cell lysates using GTX87110 MYF5 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX87110

GTX87110 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX87110 MYF5 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87110

GTX87110 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX87110 MYF5 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in Western Blot (WB). GTX87110

GTX87110 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28467493

REFERENCE

Application Reference

Migocka-Patrza?ek M et al. Int J Mol Sci 2022; 23 (15) Unique Features of River Lamprey (Lampetra fluviatilis) Myogenesis.
Hernandez-Ancheyta L et al. Int J Parasitol 2017; (Epub) Trichinella spiralis muscle larvae excretory-secretory products induce changes in cytoskeletal and myogenic transcription factors in primary myoblast cultures.
Application : WB
Reactivity : Mouse
Willkomm L et al. PLoS One 2017; 12 (5) : e0176609 p38 MAPK activation and H3K4 trimethylation is decreased by lactate in vitro and high intensity resistance training in human skeletal muscle.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

MYF5 antibody Cat. No. GTX87110

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-MYF5 antibody used in Western Blot (WB). GTX87110
Date : Anonymous submitted on 08-Jan-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : C2C12 cells
Amount used : 40μg
Blocking : 1% BSA,10%HS,0.075% Tween 20 in Tris-Cl pH7.4, 25°C, 1Hr
Primary Antibody : 1:250, 4°C, 16Hr
Lane Description : As MYF5 is a key transcription factor in myogenic specification and initial differentiation, WB was performed on C2C12 mouse myoblasts cultured in proliferation medium (PM) and then shifted to differentiation medium (DM) to allow myotubes formation.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399