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Macro H2A.2 antibody [N3C3]

Anti-Macro H2A.2 antibody [N3C3] used in Western Blot (WB). GTX116234
Anti-Macro H2A.2 antibody [N3C3] used in Western Blot (WB). GTX116234
Anti-Macro H2A.2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116234
Anti-Macro H2A.2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116234
Anti-Macro H2A.2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116234

Cat. No. GTX116234

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Macro H2A.2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Mouse brain , human H2AFY2-transfected 293T cells

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Macro H2A.2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621251

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family member Y2 , macroH2A2

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

Database

Research Area

DATA IMAGES

Anti-Macro H2A.2 antibody [N3C3] used in Western Blot (WB). GTX116234

GTX116234 WB Image

Macro H2A.2 antibody detects Macro H2A.2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human H2AFY2-transfected 293T cells
10 % SDS-PAGE
Macro H2A.2 antibody (GTX116234) dilution: 1:1000

Anti-Macro H2A.2 antibody [N3C3] used in Western Blot (WB). GTX116234

GTX116234 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX116234 diluted at 1:1000

Anti-Macro H2A.2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116234

GTX116234 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Macro H2A.2(GTX116234) antibody at 1:500 dilution.

Anti-Macro H2A.2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116234

GTX116234 IHC-P Image

Macro H2A.2 antibody [N3C3] detects Macro H2A.2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Macro H2A.2 antibody [N3C3] (GTX116234) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Macro H2A.2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116234

GTX116234 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Macro H2A.2(GTX116234) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169