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Maltose binding protein antibody [10C12]

Anti-Maltose binding protein antibody [10C12] used in (). GTX50060

Cat. No. GTX50060

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10C12

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 E. coli
Package
100 μl ($399)
See all Maltose binding protein products

APPLICATION

Application Note

We recommend the following starting dilutions:
WB-Ag: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Recombinant MBP purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

RRID

AB_11164187

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myelin basic protein

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Maltose binding protein antibody [10C12] used in (). GTX50060

GTX50060 Image

Recombinant protein MBP-tagged BCDEF, TPD52 and MAGEA3(each 20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL

REFERENCE

Application Reference

Hung PJ et al. Mol Cell 2018; 71 (2) : 332-342.e8 MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining.
Application : WB
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399