GeneTex
United States (US)

Midkine antibody [C1C3]

GTX108439_WB_19011507_918.jpg

Cat No. GTX108439

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Midkine. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ27379 antibody, MK antibody, NEGF2 antibody, MDK antibody, midgestation and kidney protein antibody, ARAP antibody, neurite outgrowth-promoting factor 2 antibody, neurite outgrowth-promoting protein antibody, amphiregulin-associated protein antibody, midkine antibody, midkine (neurite growth-promoting factor 2) antibody

Cellular Localization

Secreted

Background

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004 [PubMed 15197188]).[supplied by OMIM]
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Database

Research Area

DATA IMAGES
GTX108439_WB_19011507_918.jpg

GTX108439 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
B:Hep G2(GTX27900)
12% SDS PAGE
GTX108439 diluted at 1:200

SDS
Glycerol.pdf
Thimerosal.pdf