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Mitofilin antibody

Anti-Mitofilin antibody used in Western Blot (WB). GTX115523
Anti-Mitofilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115523

Cat. No. GTX115523

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Mitofilin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Mitofilin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166340

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inner membrane mitochondrial protein , HMP , MICOS60 , MINOS2 , Mic60 , P87 , P87/89 , P89 , PIG4 , PIG52

Cellular Localization

Mitochondrion inner membrane

Database

Research Area

DATA IMAGES

Anti-Mitofilin antibody used in Western Blot (WB). GTX115523

GTX115523 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mitofilin antibody (GTX115523) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Mitofilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115523

GTX115523 ICC/IF Image

Mitofilin antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: Mitofilin protein stained by Mitofilin antibody (GTX115523) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Adriaenssens E et al. Nat Cell Biol 2023; 25 (3) : 467-480 Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.
Application : WB
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REVIEW

Mitofilin antibody Cat. No. GTX115523

Rating
( Average 5 based on 1 users reviews)
Application
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Mitofilin antibody used in Immunoprecipitation (IP). GTX115523
Date : Anonymous submitted on 28-Jul-2016
Score :
Application Tested : IP
Sample Species : Hu
Sample : MM.1S cells
Amount used : 300μg for IP
Blocking : 5% milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Immunoprecipitation of Mitofilin protein from MM.1S whole cell extracts using 3 μg of Mitofilin antibody (GTX115523) and detected by Mitofilin antibody of WB.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169