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Mouse IL2 protein (active)

Mouse IL2 protein (active). GTX00285-pro
Mouse IL2 protein (active). GTX00285-pro
Mouse IL2 protein (active). GTX00285-pro
Mouse IL2 protein (active). GTX00285-pro

Cat No. GTX00285-pro

Application Functional Assay
Species Mouse
Package
10 μg ($249)
APPLICATION

Application Note

Interleukin-2   (IL-2), the main lymphokine produced by activated CD4 T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope. The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope. IL2 significantly decreased cell growth of 3T3 cells, and the ED₅₀ for this effect is typically 9. 876-13. 35 ng/ml.

Observed MW

25 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala21~Gln169 (NP_032392.1)

Expression System

293F cell

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Mouse IL2 protein (active). GTX00285-pro

GTX00285-pro Functional Assay Image

The dose effect curve of GTX00285-pro Mouse IL2 protein (active) on 3T3 cells. After incubation of Mouse IL2 protein (active) for 48 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Mouse IL2 protein (active). GTX00285-pro

GTX00285-pro Functional Assay Image

The inhibition effect of GTX00285-pro Mouse IL2 protein (active) on cell proliferation.
(A) Unstimulated 3T3 cells cultured in DMEM for 72hrs.
(B) 3T3 cells cultured in DMEM, stimulated with IL2 (10 ng/ml) for 72hrs.

Mouse IL2 protein (active). GTX00285-pro

GTX00285-pro Image

SDS-PAGE analysis of GTX00285-pro Mouse IL2 protein (active).

Mouse IL2 protein (active). GTX00285-pro

GTX00285-pro Image

WB analysis of GTX00285-pro Mouse IL2 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 249