GeneTex
United States (US)

Mouse IgA antibody [RM220]

GTX20289_20160517_ELISA_1_18121410_897.jpg
GTX20289_20160517_WB_18121410_728.jpg
GTX20289_20160517_ELISA_18121410_968.jpg

Cat No. GTX20289

Host Rabbit
Clonality Monoclonal
Clone Name RM220
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity Mouse
APPLICATION

Application Note

ELISA: 0.005 ug/mL – 0.2 ug/mL, Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL , Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL , Western Blot (WB): 0.5 ug/mL-2 ug/mL.

Specificity/Sensitivity

This antibody specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. This antibody will bind to secreted Mouse IgA, it has not been confirmed whether it binds to non-secreted IgA or not.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

secreted mouse IgA

Target Isotype

IgA

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

immunoglobulin heavy constant alpha Antibody , Igha Antibody
DATA IMAGES
GTX20289_20160517_ELISA_1_18121410_897.jpg

GTX20289 ELISA Image

A titer ELISA of mouse IgA. The plate was coated with different amount of mouse IgA. A serial dilution of RM220 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

GTX20289_20160517_WB_18121410_728.jpg

GTX20289 WB Image

Western blot of nonreduced(-) and reduced(+) mouse IgA, using 0.5ug/mL of RevMAb clone RM220. This antibody reacts to nonreduced IgA.

GTX20289_20160517_ELISA_18121410_968.jpg

GTX20289 ELISA Image

ELISA of mouse immunoglobulins shows RM220 reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a,IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Package List Price ($)
$ 299