GeneTex
United States (US)

Mouse IgG antibody [RM104]

GTX20295_20160517_ELISA_18121410_291.jpg
GTX20295_20160517_ELISA_1_18121410_310.jpg
GTX20295_20160517_WB_18121410_550.jpg

Cat No. GTX20295

Host Rabbit
Clonality Monoclonal
Clone Name RM104
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity Mouse
APPLICATION

Application Note

ELISA: 0.005 ug/mL-0.2 ug/mL, Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL, Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL, Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.

Specificity/Sensitivity

This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG

Target Isotype

IgG

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ighg1 Antibody , immunoglobulin heavy constant gamma 1 (G1m marker) Antibody
DATA IMAGES
GTX20295_20160517_ELISA_18121410_291.jpg

GTX20295 ELISA Image

ELISA of mouse immunoglobulins shows RM104reacts to the Fc region of mouse IgG1, IgG2a,IgG2b, and IgG3; no cross reactivity with IgM,IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondary antibody.

GTX20295_20160517_ELISA_1_18121410_310.jpg

GTX20295 ELISA Image

A titer ELISA of mouse IgG. The plate was coatedwith different amounts of mouse IgG. A serial dilutionof RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

GTX20295_20160517_WB_18121410_550.jpg

GTX20295 WB Image

Western blot of nonreduced(-) and reduced(+)mouse immunoglobulins (20ng/lane), using 0.2ug/mL of clone RM104. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b,and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.

Package List Price ($)
$ 219