GeneTex
United States (US)

Mouse IgG2a antibody [RM219]

GTX20293_20160517_ELISA_1_18121410_479.jpg
GTX20293_20160517_WB_18121410_994.jpg
GTX20293_20160517_ELISA_18121410_244.jpg

Cat No. GTX20293

Host Rabbit
Clonality Monoclonal
Clone Name RM219
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity Mouse
APPLICATION

Application Note

ELISA: 0.005 ug/mL – 0.2 ug/mL, Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL, Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL, Western Blot (WB): 0.5 ug/mL-2 ug/mL.

Specificity/Sensitivity

This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG2a

Target Isotype

IgG2a

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

immunoglobulin heavy constant gamma 2A Antibody , Ighg2a Antibody
DATA IMAGES
GTX20293_20160517_ELISA_1_18121410_479.jpg

GTX20293 ELISA Image

A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugatedanti-rabbit IgG as the secondary antibody.

GTX20293_20160517_WB_18121410_994.jpg

GTX20293 WB Image

Western blot of nonreduced(-) and reduced(+)mouse IgG2a, using 0.5ug/mL of RevMAb cloneRM219. This antibody reacts to nonreducedIgG2a (~150 kDa).

GTX20293_20160517_ELISA_18121410_244.jpg

GTX20293 ELISA Image

ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coatedwith 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was usedas the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondaryantibody.

Package List Price ($)
$ 289