GeneTex
  • Country / Location Selection

United States (US)

Mouse IgG2b antibody [RM108]

Mouse IgG2b antibody [RM108]. GTX60855
Mouse IgG2b antibody [RM108]. GTX60855
Mouse IgG2b antibody [RM108]. GTX60855

Cat No. GTX60855

Host Rabbit
Clonality Monoclonal
Clone Name RM108
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity Mouse
Package
100 μg ($219)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
PROPERTIES

Form

Liquid

Buffer

PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG2b

Target Isotype

IgG2b

Purification

Protein A affinity purified from an animal origin–free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ighg1 Antibody , Igh-4 Antibody , VH7183 Antibody , immunoglobulin heavy constant gamma 1 (G1m marker) Antibody , IgG1 Antibody
DATA IMAGES
Mouse IgG2b antibody [RM108]. GTX60855

GTX60855 ELISA Image

ELISA of mouse immunoglobulins shows RM108 reacted to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Mouse IgG2b antibody [RM108]. GTX60855

GTX60855 WB Image

Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/mL of clone RM108. This antibody reacted to nonreduced IgG2b (~150 kDa) much stronger than the reduced γ2b(~50 kDa).

Mouse IgG2b antibody [RM108]. GTX60855

GTX60855 ELISA Image

A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

REFERENCE
There are currently no references for Mouse IgG2b antibody [RM108] (GTX60855). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Mouse IgG2b antibody [RM108] (GTX60855). Be the first to share your experience with this product.
Package List Price ($)
$ 219