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Mouse Wnt5a protein (active)

Mouse Wnt5a protein (active). GTX00301-pro
Mouse Wnt5a protein (active). GTX00301-pro
Mouse Wnt5a protein (active). GTX00301-pro

Cat No. GTX00301-pro

Application Functional Assay
Species Mouse
Package
10 μg ($329)
APPLICATION

Application Note

Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) is a ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Besides, WNT Inhibitory Factor 1 (WIF1) has been identified as an interactor of WNT5A, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse WNT5A and recombinant rat WIF1. Briefly, WNT5A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to WIF1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-WNT5A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of WNT5A and WIF1 was in a dose dependent manner.

Observed MW

62 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal GST-Tag; Ile62~Lys380 (NP_001243153.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

wingless-type MMTV integration site family, member 5A , 8030457G12Rik , Wnt-5a

Database

Research Area

DATA IMAGES
Mouse Wnt5a protein (active). GTX00301-pro

GTX00301-pro Functional Assay Image

Functional ELISA analysis of GTX00301-pro Mouse Wnt5a protein (active) which can bind immobilized WIF1 protein.

Mouse Wnt5a protein (active). GTX00301-pro

GTX00301-pro Image

SDS-PAGE analysis of GTX00301-pro Mouse Wnt5a protein (active).

Mouse Wnt5a protein (active). GTX00301-pro

GTX00301-pro Image

WB analysis of GTX00301-pro Mouse Wnt5a protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 329